Wilcox M H, Winstanley T G, Spencer R C
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, UK.
J Antimicrob Chemother. 1994 Mar;33(3):431-41. doi: 10.1093/jac/33.3.431.
Both teicoplanin and vancomycin were found to bind to a range of polymer surfaces. The binding of teicoplanin to specimen vessel surfaces was, on average, four times greater than that of vancomycin and was particularly marked with silconized polymers (5.2 micrograms/cm2). Pre-exposure of a polymer surface to human body fluids caused a 60% reduction in teicoplanin binding. Reduction of the negative surface charge on a polymer surface with ferric nitrate resulted in a ten-fold increase in teicoplanin binding. The accumulation of a strain of Staphylococcus epidermidis on silicone rubber catheter segments pre-exposed to glycopeptide antibiotics was examined. In phosphate buffered saline binding of bacteria to vancomycin-treated polymer was greater than to an unexposed control surface. In contrast, in human serum both antibiotics caused reductions in adherent growth. The binding of glycopeptide antibiotics, in particular teicoplanin, to polymer surfaces may interfere with the results of in-vitro assays. However, this phenomenon may be useful in the prevention of bacterial accumulation on the surfaces of medical devices.
发现替考拉宁和万古霉素均能与一系列聚合物表面结合。替考拉宁与标本容器表面的结合平均比万古霉素高四倍，在硅化聚合物上尤为明显（5.2微克/平方厘米）。聚合物表面预先暴露于人体体液会使替考拉宁的结合减少60%。用硝酸铁降低聚合物表面的负表面电荷会使替考拉宁的结合增加十倍。研究了一株表皮葡萄球菌在预先暴露于糖肽类抗生素的硅橡胶导管段上的积聚情况。在磷酸盐缓冲盐水中，细菌与万古霉素处理过的聚合物的结合大于与未暴露的对照表面的结合。相反，在人血清中，两种抗生素都会导致粘附生长减少。糖肽类抗生素，特别是替考拉宁与聚合物表面的结合可能会干扰体外试验的结果。然而，这种现象可能有助于预防细菌在医疗设备表面的积聚。
