完整红细胞中带3蛋白侧向和旋转运动性的差异调控
Differential control of band 3 lateral and rotational mobility in intact red cells.
作者信息
Corbett J D, Agre P, Palek J, Golan D E
机构信息
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
J Clin Invest. 1994 Aug;94(2):683-8. doi: 10.1172/JCI117385.
Abstract
Measurements of integral membrane protein lateral mobility and rotational mobility have been separately used to investigate dynamic protein--protein and protein-lipid interactions that underlie plasma membrane structure and function. In model bilayer membranes, the mobilities of reconstituted proteins depend on the size of the diffusing molecule and the viscosity of the lipid bilayer. There are no direct tests, however, of the relationship between mechanisms that control protein lateral mobility and rotational mobility in intact biological membranes. We have measured the lateral and rotational mobility of band 3 in spectrin-deficient red blood cells from patients with hereditary spherocytosis and hereditary pyropoikilocytosis. Our data suggest that band 3 lateral mobility is regulated by the spectrin content of the red cell membrane. In contrast, band 3 rotational mobility is unaffected by changes in spectrin content. Band 3 lateral mobility and rotational mobility must therefore be controlled by different molecular mechanisms.
摘要
整合膜蛋白侧向迁移率和旋转迁移率的测量已分别用于研究构成质膜结构和功能基础的动态蛋白-蛋白及蛋白-脂质相互作用。在模型双层膜中，重组蛋白的迁移率取决于扩散分子的大小和脂质双层的黏度。然而，对于完整生物膜中控制蛋白侧向迁移率和旋转迁移率的机制之间的关系，尚无直接测试。我们测量了遗传性球形红细胞增多症和遗传性热异形红细胞增多症患者的血影蛋白缺陷红细胞中带3蛋白的侧向和旋转迁移率。我们的数据表明，带3蛋白的侧向迁移率受红细胞膜血影蛋白含量的调节。相比之下，带3蛋白的旋转迁移率不受血影蛋白含量变化的影响。因此，带3蛋白的侧向迁移率和旋转迁移率必定受不同分子机制的控制。
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