Dillon P W, Keefer K, Blackburn J H, Houghton P E, Krummel T M
Department of Surgery, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033.
J Surg Res. 1994 Jul;57(1):170-3. doi: 10.1006/jsre.1994.1126.
Adhesive interactions between lymphocytes and components of the extracellular matrix (ECM) within a wound environment play a crucial role in determining the inflammatory response following tissue injury. In fetal wounds the extracellular matrix is composed predominantly of hyaluronic acid. Within this environment the inflammatory reaction as a result of injury is minimal. We propose that this lack of an inflammatory cell response in the fetal wound is due to the high levels of hyaluronic acid within the ECM and the inability of lymphocytes to adhere to this matrix component. Therefore, we examined the adhesive properties of fetal lymphocytes to fibronectin, vitronectin, collagen types I, III, IV, V, and hyaluronic acid--ECM components involved in fetal and adult wound environments. Fetal lymphocytes from both spleen and thymus demonstrated significant binding capabilities to fibronectin, vitronectin, and collagen types I and III. No intrinsic binding capabilities were detected to hyaluronic acid. Adhesion was not affected by the addition of IL-1, IFN-gamma, or phorbol dibutyrate. The inability of lymphocytes to adhere to hyaluronic acid helps to explain the lack of inflammation found in fetal wounds and serves to demonstrate the importance of ECM-lymphocyte interactions in determining the inflammatory response during fetal wound healing.
在伤口环境中，淋巴细胞与细胞外基质（ECM）成分之间的黏附相互作用在决定组织损伤后的炎症反应中起着关键作用。在胎儿伤口中，细胞外基质主要由透明质酸组成。在这种环境下，损伤引起的炎症反应很轻微。我们认为，胎儿伤口中缺乏炎症细胞反应是由于ECM中透明质酸水平较高，以及淋巴细胞无法黏附于这种基质成分。因此，我们研究了胎儿淋巴细胞对纤连蛋白、玻连蛋白、I型、III型、IV型、V型胶原蛋白以及透明质酸（胎儿和成人伤口环境中涉及的ECM成分）的黏附特性。来自脾脏和胸腺的胎儿淋巴细胞对纤连蛋白、玻连蛋白以及I型和III型胶原蛋白表现出显著的结合能力。未检测到对透明质酸的固有结合能力。添加白细胞介素-1、γ-干扰素或佛波酯二丁酸酯对黏附没有影响。淋巴细胞无法黏附于透明质酸有助于解释胎儿伤口中炎症的缺乏，并证明了ECM-淋巴细胞相互作用在决定胎儿伤口愈合过程中的炎症反应方面的重要性。
