小肠结肠炎耶尔森菌产生的热稳定脱氧核糖核酸酶（DNase）对聚合酶链反应（PCR）产物的降解作用。
Degradation of a polymerase chain reaction (PCR) product by heat-stable deoxyribonuclease (DNase) produced from Yersinia enterocolitica.
作者信息
Nakajima H, Itoh K, Arakawa E, Inoue M, Mori T, Watanabe H
机构信息
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
出版信息
Microbiol Immunol. 1994;38(2):153-6. doi: 10.1111/j.1348-0421.1994.tb01757.x.
When crude deoxyribonucleic acid (DNA) preparations by boiling were used for the polymerase chain reaction (PCR) from pathogenic and non-pathogenic Yersinia enterocolitica strains, the amplified products were degraded after their storage at 4 C. The degradation of products was prevented by the addition of ethylenediaminetetraacetate (EDTA) or treatment with proteinase K. These findings indicate that Y. enterocolitica produced heat-stable deoxyribonuclease (DNase). Proteinase K treatment would be recommended to prevent heat-stable DNase contamination in the DNA preparations for PCR from Y. enterocolitica strains.
当使用通过煮沸获得的粗制脱氧核糖核酸（DNA）制剂对致病性和非致病性小肠结肠炎耶尔森菌菌株进行聚合酶链反应（PCR）时，扩增产物在4℃储存后会降解。通过添加乙二胺四乙酸（EDTA）或用蛋白酶K处理可防止产物降解。这些发现表明小肠结肠炎耶尔森菌产生了热稳定的脱氧核糖核酸酶（DNase）。对于来自小肠结肠炎耶尔森菌菌株的用于PCR的DNA制剂，建议使用蛋白酶K处理以防止热稳定DNase污染。
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