Prasad A V, Pilcher W H, Joseph S A
Division of Neurosurgery, University of Rochester Medical Center, NY 14642.
Neurosci Lett. 1994 Mar 28;170(1):145-8. doi: 10.1016/0304-3940(94)90260-7.
The DNA-binding protein nuclear factor-kappa B (NF-kappa B) is a pleiotropic transcription factor which regulates the transcription of specific target genes such as cytokines. The existence of NF-kappa B has not been reported in brain tissue. This is the first report demonstrating the expression of NF-kappa B in the rat brain. After pentylene tetrazole (s.c.)-induced clonic-tonic seizures at an LD50 dose of 85 mg/kg, we have shown a gradual increase in NF-kappa B expression reaching a maximum at 24 h, a decrease at 48 h and again increased at 96 and 120 h. A similar time-dependent pattern was observed for the NF-kappa B subunit p50 expression. The NF-kappa B subunit p65 was not expressed at all. These data suggest a possible underlying mechanism of signal transduction and transcriptional regulation of late-response genes after perturbations in the CNS milieu.
DNA结合蛋白核因子-κB（NF-κB）是一种多效性转录因子，可调节特定靶基因（如细胞因子）的转录。在脑组织中尚未报道过NF-κB的存在。这是首次报道在大鼠脑中发现NF-κB的表达。在以85 mg/kg的半数致死剂量腹腔注射戊四氮诱导阵挛-强直发作后，我们发现NF-κB表达逐渐增加，在24小时达到最大值，48小时下降，96小时和120小时再次增加。NF-κB亚基p50的表达也观察到类似的时间依赖性模式。而NF-κB亚基p65根本不表达。这些数据提示了中枢神经系统环境受到干扰后晚期反应基因信号转导和转录调控的一种可能潜在机制。
