Dobbs D L, Shaiu W L, Benbow R M
Department of Zoology and Genetics, Iowa State University, Ames 50011.
Nucleic Acids Res. 1994 Jul 11;22(13):2479-89. doi: 10.1093/nar/22.13.2479.
We have postulated that chromosomal replication origin regions in eukaryotes have in common clusters of certain modular sequence elements (Benbow, Zhao, and Larson, BioEssays 14, 661-670, 1992). In this study, computer analyses of DNA sequences from six origin regions showed that each contained one or more potential initiation regions consisting of a putative DUE (DNA unwinding element) aligned with clusters of SAR (scaffold associated region), and ARS (autonomously replicating sequence) consensus sequences, and pyrimidine tracts. The replication origins analyzed were from the following loci: Tetrahymena thermophila macronuclear rDNA gene, Chinese hamster ovary dihydrofolate reductase amplicon, human c-myc proto-oncogene, chicken histone H5 gene, Drosophila melanogaster chorion gene cluster on the third chromosome, and Chinese hamster ovary rhodopsin gene. The locations of putative initiation regions identified by the computer analyses were compared with published data obtained using diverse methods to map initiation sites. For at least four loci, the potential initiation regions identified by sequence analysis aligned with previously mapped initiation events. A consensus DNA sequence, WAWTTDDWWWDHWGWHMAWTT, was found within the potential initiation regions in every case. An additional 35 kb of combined flanking sequences from the six loci were also analyzed, but no additional copies of this consensus sequence were found.
我们曾假设真核生物中的染色体复制起始区域具有某些模块化序列元件的共同簇（本博、赵和拉尔森，《生物论文》14卷，661 - 670页，1992年）。在本研究中，对六个起始区域的DNA序列进行计算机分析表明，每个区域都包含一个或多个潜在起始区域，这些区域由一个假定的DUE（DNA解旋元件）与SAR（支架相关区域）簇、ARS（自主复制序列）共有序列以及嘧啶序列对齐组成。所分析的复制起始点来自以下基因座：嗜热四膜虫大核rDNA基因、中国仓鼠卵巢二氢叶酸还原酶扩增子、人c - myc原癌基因、鸡组蛋白H5基因、果蝇第三染色体上的绒毛膜基因簇以及中国仓鼠卵巢视紫红质基因。将计算机分析确定的潜在起始区域的位置与使用多种方法绘制起始位点所获得的已发表数据进行了比较。对于至少四个基因座，通过序列分析确定的潜在起始区域与先前绘制的起始事件对齐。在每种情况下，在潜在起始区域内都发现了一个共有DNA序列WAWTTDDWWWDHWGWHMAWTT。还对来自这六个基因座的另外35 kb的组合侧翼序列进行了分析，但未发现该共有序列的其他拷贝。
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