Kessel D, Morgan A
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
Photochem Photobiol. 1994 May;59(5):547-9. doi: 10.1111/j.1751-1097.1994.tb02981.x.
The photobiologic properties of a chlorin-thiobarbiturate conjugate were examined. In addition to the characteristic 670 nm chlorin absorbance, the conjugate exhibited new absorbance bands at 517 and 743 nm, but these were not a part of the fluorescence emission spectra, nor was fluorescence observed at wavelengths > 670 nm. The action spectrum indicated that the long-wavelength absorbance of the conjugate was not involved in phototoxicity in cell culture, and that only irradiation at wavelengths associated with the chlorin moiety yielded a cytotoxic effect. In vivo cytotoxicity elicited by 745 nm irradiation is attributed to thermal effects.
对二氢卟吩-硫代巴比妥酸共轭物的光生物学特性进行了研究。除了二氢卟吩特征性的670nm吸光度外，该共轭物在517和743nm处还呈现出新的吸收带，但这些吸收带并非荧光发射光谱的一部分，在波长>670nm处也未观察到荧光。作用光谱表明，该共轭物的长波长吸收与细胞培养中的光毒性无关，只有与二氢卟吩部分相关波长的照射才会产生细胞毒性作用。745nm照射引起的体内细胞毒性归因于热效应。
