Murawaki Y, Yamamoto H, Koda M, Kawasaki H
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Res Commun Chem Pathol Pharmacol. 1994 Apr;84(1):63-72.
To examine whether serum collagenase activity reflects the amount of collagenase activity in the fibrotic liver of rats, we simultaneously measured serum and hepatic collagenase activities in rats with carbon tetrachloride-induced liver injury. Serum collagenase was measured after reactivation by denaturing and dissociating the inhibitors with potassium thiocyanate and aminophenylmercuric acetate, while hepatic collagenase was measured after the removal of plasma protein and activation with aminophenylmercuric acetate. Serum and hepatic collagenase activities increased in the carbon tetrachloride-treated rats with the progression of liver fibrosis, and both activities of collagenase were closely correlated each other. These results suggest that serum collagenase activity measured in these assay conditions could be used as a noninvasive marker for hepatic collagenolysis in carbon tetrachloride-treated rats.
为了研究血清胶原酶活性是否反映大鼠纤维化肝脏中胶原酶活性的量，我们同时测量了四氯化碳诱导肝损伤大鼠的血清和肝脏胶原酶活性。血清胶原酶是在通过硫氰酸钾和氨基苯基汞醋酸盐使抑制剂变性和解离而重新激活后进行测量的，而肝脏胶原酶是在去除血浆蛋白并用氨基苯基汞醋酸盐激活后进行测量的。随着肝纤维化的进展，四氯化碳处理的大鼠血清和肝脏胶原酶活性增加，并且两种胶原酶活性彼此密切相关。这些结果表明，在这些测定条件下测量的血清胶原酶活性可作为四氯化碳处理大鼠肝胶原分解的非侵入性标志物。
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