Hakim S, Merguerian P A, Chavez D R
Department of Surgery, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire.
Urology. 1994 Jul;44(1):139-42. doi: 10.1016/s0090-4295(94)80028-6.
To create an improved delivery vehicle for cultured uroepithelial cells using biodegradable mesh and collagen gel.
Twenty uroepithelial grafts were prepared by seeding cultured urothelial cells onto previously prepared collagen gel-polyglactin mesh. As a control, cells were seeded onto 20 plain polyglactin mesh (without gel) squares in the same fashion.
The presence of urothelial cells was confirmed in all cultures by immunohistochemistry testing with anticytokeratin antibody. After 5 to 7 days, a confluent monolayer of cells covered 90% to 100% of the surface of all 20 collagen gel-mesh grafts. A confluent monolayer of cells did not form on any control graft (plain mesh). After 3 weeks, the cells had formed a sporadic, multiple cell layer confluence over 10% to 50% of the control graft surface; however, the mesh became friable and fell apart.
By combining collagen gel with a biodegradable mesh scaffold, we created a surgically implantable cultured uroepithelial graft. The production of the collagen gel-mesh vehicle is simple and inexpensive. With this technique, the use of cultured urothelium for reconstructive urologic surgery can be investigated in greater detail.
使用可生物降解网片和胶原凝胶创建一种改良的培养尿路上皮细胞递送载体。
通过将培养的尿路上皮细胞接种到预先制备的胶原凝胶-聚乙醇酸网片上制备20个尿路上皮移植物。作为对照，以相同方式将细胞接种到20个普通聚乙醇酸网片（无凝胶）方块上。
通过抗细胞角蛋白抗体免疫组织化学检测在所有培养物中均证实存在尿路上皮细胞。5至7天后，汇合的单层细胞覆盖了所有20个胶原凝胶-网片移植物表面的90%至100%。在任何对照移植物（普通网片）上均未形成汇合的单层细胞。3周后，细胞在对照移植物表面的10%至50%上形成了散在的多层细胞汇合；然而，网片变得易碎并散开。
通过将胶原凝胶与可生物降解网片支架相结合，我们创建了一种可手术植入的培养尿路上皮移植物。胶原凝胶-网片载体的制备简单且成本低廉。利用这项技术，可以更详细地研究培养的尿路上皮在重建性泌尿外科手术中的应用。
