Atala A, Freeman M R, Vacanti J P, Shepard J, Retik A B
Pediatric Urology Research Laboratory, Children's Hospital, Boston, Massachusetts.
J Urol. 1993 Aug;150(2 Pt 2):608-12. doi: 10.1016/s0022-5347(17)35561-1.
The harvest of human bladder muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human bladder urothelial and muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and bladder muscle cells, when implanted on polyglycolic acid fibers, from new urological structures in vivo composed of both cell types. The human cell-polymer xenografts can be recovered from host animals at extended times after implantation. These data suggest that feasibility of using polyglycolic acid polymers as substrates for the creation of human urothelial and muscle grafts for genitourinary reconstruction.
获取人类膀胱肌肉和尿路上皮细胞并使其后续生长，可能对泌尿生殖系统重建中的组织替代有用。我们之前报道了一种利用可生物降解聚合物在体内获取、输送和培养兔尿路上皮的系统。现在，我们已对该系统进行扩展和调整，用于获取和在体内植入人类膀胱尿路上皮细胞和肌肉细胞。聚乙醇酸合成聚合物纤维可作为支架和输送载体，用于将兔尿路上皮细胞植入无胸腺宿主动物体内。这些聚合物在体内会缓慢降解，使尿路上皮细胞能在植入部位存活。我们证明聚乙醇酸聚合物能支持兔尿路上皮细胞在原位增殖，并可作为一种可塑性基质来创建替代降解聚合物纤维的新泌尿结构。我们还表明，人类尿路上皮细胞和膀胱肌肉细胞植入聚乙醇酸纤维后，在体内会形成由这两种细胞类型组成的新泌尿结构。植入后较长时间可从宿主动物体内回收人类细胞 - 聚合物异种移植物。这些数据表明，使用聚乙醇酸聚合物作为基质来创建用于泌尿生殖系统重建的人类尿路上皮和肌肉移植物具有可行性。
