Mikhail M M, Waslien C I, Gabr M K, Mansour M M
Am J Clin Nutr. 1975 Mar;28(3):233-7. doi: 10.1093/ajcn/28.3.233.
The in vitro uptake of 14C-glycine, 14C-leucine and 35S-methionine by red blood cells as well as the red blood cells and plasma amino acid patterns were studied in 20 children with protein-calorie malnutrition. Blood samples were obtained from each patient on admission, following clinical recovery and, when possible, several months later. Amino acid uptake as well as levels of red blood cells and plasma amino acids are quantitatively changed in protein-calorie malnutrition. Significantly increased uptake of 14C-glycine but decreased uptake of 14C-leucine and 35S-methionine were observed in patients on admission. Following clinical recovery the uptake values of all three amino acids were markedly reduced to levels significantly below normal. Several months later values for glycine and leucine uptake were found to be normal. No significant correlation, however, was found between glycine, leucine, or methionine uptake values and concentrations of these amino groups in RBCs or plasma of patients. It is concluded that the elevations observed in red blood cell amino acids of protein-calorie malnutrition patients are not purely a result of increased amino acid uptake of RBCs and that normalization of both amino acid uptake values and their concentrations in RBCs require longer than the treatment period.
对20名蛋白质 - 热量营养不良的儿童，研究了红细胞对¹⁴C - 甘氨酸、¹⁴C - 亮氨酸和³⁵S - 蛋氨酸的体外摄取情况，以及红细胞和血浆氨基酸模式。在每位患者入院时、临床康复后以及尽可能在数月后采集血样。在蛋白质 - 热量营养不良中，氨基酸摄取以及红细胞和血浆氨基酸水平发生了定量变化。入院时患者¹⁴C - 甘氨酸摄取显著增加，但¹⁴C - 亮氨酸和³⁵S - 蛋氨酸摄取减少。临床康复后，所有三种氨基酸的摄取值均显著降低至明显低于正常水平。数月后，甘氨酸和亮氨酸摄取值恢复正常。然而，患者红细胞或血浆中甘氨酸、亮氨酸或蛋氨酸的摄取值与这些氨基的浓度之间未发现显著相关性。得出的结论是，蛋白质 - 热量营养不良患者红细胞氨基酸升高并非单纯由于红细胞氨基酸摄取增加所致，并且红细胞中氨基酸摄取值及其浓度的正常化所需时间长于治疗期。
