Diemer T, Weidner W, Michelmann H W, Nierste B, Ringert R H
Department of Urology, Georgia-Augusta University, Göttingen, Germany.
Andrologia. 1994 Mar-Apr;26(2):93-6. doi: 10.1111/j.1439-0272.1994.tb00764.x.
This paper describes an attempt to establish a new combined method of leukocyte analysis in human ejaculate by Percoll density gradient centrifugation and consecutive flow cytometry. As a first step, leukocyte separation was performed by Percoll density gradient centrifugation with consecutive enrichment of leukocytes, especially granulocytes, in the 40%/60% and 60%/80% Percoll interfaces. Then these fractions were stained with specific monoclonal antibodies and analysed in a Facscan flow cytometer. Flow cytometric analysis did not demonstrate identifiable leukocyte populations, indicating a questionable cross-reaction with spermatozoal elements. Therefore, the combined technique of Percoll density gradient centrifugation and flow cytometric analysis were considered unsuitable for clinical leukocyte determination.
本文描述了一种尝试，即通过Percoll密度梯度离心法和连续流式细胞术建立一种新的人类精液白细胞分析联合方法。第一步，通过Percoll密度梯度离心法进行白细胞分离，在40%/60%和60%/80%的Percoll界面连续富集白细胞，尤其是粒细胞。然后用特异性单克隆抗体对这些组分进行染色，并在流式细胞仪上进行分析。流式细胞术分析未显示可识别的白细胞群体，表明与精子成分存在可疑的交叉反应。因此，Percoll密度梯度离心法和流式细胞术分析的联合技术被认为不适用于临床白细胞测定。
