85 kDa磷脂酶A2的酰基链选择性及完整巨噬细胞中的释放过程
Acyl-chain selectivity of the 85 kDa phospholipase A2 and of the release process in intact macrophages.
作者信息
Sundler R, Winstedt D, Wijkander J
机构信息
Department of Medical and Physiological Chemistry, Lund University, Sweden.
出版信息
Biochem J. 1994 Jul 15;301 ( Pt 2)(Pt 2):455-8. doi: 10.1042/bj3010455.
Abstract
The selectivity of the intracellular 85 kDa phospholipase A2 (PLA2-85) towards fatty acids closely related to arachidonic acid has been investigated, using purified PLA2-85 from J774 cells and mixed phospholipids, dually acyl-chain-labelled in the sn-2 position. In parallel experiments, we assessed the acyl-chain selectivity of the release process in intact, dually labelled, peritoneal mouse macrophages responding to either calcium ionophore or zymosan beads in the presence of indomethacin and BSA. The results obtained in the two systems were very similar, which supports previous evidence that PLA2-85 is responsible for stimulus-induced release of eicosanoid precursor in mouse macrophages. In the in vitro system, PLA2-85 was found to exhibit a moderate selectivity towards C20 acyl chains differing in double-bond structure, while the sensitivity to acyl-chain length was more pronounced. Together with previous data, these results demonstrate a striking preference for C20 over either C18 or C22 unsaturated acyl chains.
摘要
利用从J774细胞中纯化得到的85 kDa细胞内磷脂酶A2（PLA2-85）和在sn-2位进行双酰基链标记的混合磷脂，研究了PLA2-85对与花生四烯酸密切相关的脂肪酸的选择性。在平行实验中，我们评估了在吲哚美辛和牛血清白蛋白存在的情况下，完整的、双标记的腹膜小鼠巨噬细胞对钙离子载体或酵母聚糖珠作出反应时释放过程的酰基链选择性。在这两个系统中获得的结果非常相似，这支持了先前的证据，即PLA2-85负责刺激诱导的小鼠巨噬细胞中类花生酸前体的释放。在体外系统中，发现PLA2-85对双键结构不同的C20酰基链表现出适度的选择性，而对酰基链长度的敏感性更为明显。与先前的数据一起，这些结果表明，与C18或C22不饱和酰基链相比，PLA2-85对C20酰基链具有明显的偏好。
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