Boon M E, van der Poel H G, Tan C J, Kok L P
Leiden Cytology and Pathology Laboratory, The Netherlands.
Anal Quant Cytol Histol. 1994 Apr;16(2):131-6.
The influence of fixation and embedding methods in seven urologic tumor samples was studied karyometrically for 12 preparatory techniques. Routine histologic formalin fixation was compared with Carbowax and Kryofix fixatives. Also, histologic material was studied embedded in paraffin and plastic (GMA) and secondarily in plastic (after paraffin embedding). From the cell suspensions, cytocentrifuge preparations were made (10 minutes, 1,500 rpm). The influence on nuclear light microscopic characteristics for two morphometric and five texture features in Feulgen-stained nuclei was evaluated. Nuclear features were influenced by both fixation and embedding method. The most extensive karyometric differences were found between different embedding techniques. More abnormally shaped tumor cell nuclei tended to occur with plastic (GMA) embedding methods. Plastic embedding proved to be superior to paraffin for all three fixation methods. Reembedding of archival formalin-fixed, paraffin-embedded material in plastic greatly influenced nuclear features, nuclear area in particular. We conclude that nuclear features are particularly influenced by the embedding method applied.
采用12种制备技术，通过细胞核测量法研究了七种泌尿系统肿瘤样本中固定和包埋方法的影响。将常规组织学福尔马林固定与聚乙二醇和低温固定剂进行了比较。此外，还研究了包埋在石蜡和塑料（GMA）中的组织学材料，以及二次包埋在塑料中的材料（石蜡包埋后）。从细胞悬液中制备细胞离心涂片（10分钟，1500转/分钟）。评估了对福尔根染色细胞核中两个形态计量学特征和五个纹理特征的核光镜特征的影响。核特征受固定和包埋方法的影响。在不同的包埋技术之间发现了最广泛的细胞核测量差异。采用塑料（GMA）包埋方法时，更多异常形状的肿瘤细胞核倾向于出现。对于所有三种固定方法，塑料包埋都被证明优于石蜡包埋。将存档的福尔马林固定、石蜡包埋材料重新包埋在塑料中对核特征有很大影响，尤其是核面积。我们得出结论，核特征特别受所应用的包埋方法的影响。
