Benchaib M, Souchier C, Ffrench M, Remy F, Bryon P A
Analytical Cytology Laboratory, University Claude Bernard, Lyon, France.
Anal Quant Cytol Histol. 1994 Apr;16(2):137-44.
The DNA content of plasma cells was estimated by fluorescence image analysis in monoclonal gammopathies. Image analysis provides the possibility of measuring the bone marrow plasma cell population separately from the other myeloid cells. Plasma cells were labeled, identified and then relocated for propidium iodide intensity measurements. Patients with DNA malignancy grade > 0.20 or with DNA index > 1.15 had a poorer survival rate than did patients with a low DNA malignancy grade or DNA index. DNA malignancy grade is based on the variance of DNA values around the normal diploid peak, as proposed by Böcking. Of all the biologic and clinical parameters investigated, the serum beta 2 microglobulin level was the most important prognostic factor for survival in the multivariate regression analysis, and the DNA entropy index provided the most significant additional information.
通过荧光图像分析评估单克隆丙种球蛋白病中浆细胞的DNA含量。图像分析提供了将骨髓浆细胞群体与其他髓系细胞分开测量的可能性。对浆细胞进行标记、识别，然后重新定位以测量碘化丙啶强度。DNA恶性度分级>0.20或DNA指数>1.15的患者生存率低于DNA恶性度分级或DNA指数较低的患者。DNA恶性度分级基于Böcking提出的正常二倍体峰周围DNA值的方差。在所有研究的生物学和临床参数中，血清β2微球蛋白水平是多变量回归分析中生存的最重要预后因素，而DNA熵指数提供了最显著的额外信息。
