大戟科成分12. 通讯(1)。从大狼毒中分离并鉴定四种新的脑苷脂的结构

Constituents of euphorbiaceae 12. Comm. (1). Isolation and structure elucidation of four new cerebrosides from Euphorbia biglandulosa Desf.

作者信息

Falsone G, Cateni F, Visintin G, Lucchini V, Wagner H, Seligmann O

机构信息

Department of Pharmaceutical Sciences, University of Trieste.

出版信息

Farmaco. 1994 Mar;49(3):167-74.

PMID:8043167

Abstract

Four new cerebrosides 4-6 were isolated from the latex of Euphorbia biglandulosa Desf. and their structures determined. Normal and reverse-phase flash chromatography was effective for the isolation of the cerebrosides, and FAB-MS spectrometry, 1H-NMR, 13C-NMR, DQF-COSY and HMQC experiments and chemical reactions were useful in elucidating their structure. EI-MS of the dimethyl disulfide derivatives of the long chain bases and glucosphingoside-heptaacetates was decisive for the determination of the double bond position on the long chain parts.

摘要

从大戟科大戟属植物大花大戟的乳汁中分离出四种新的脑苷脂4 - 6，并确定了它们的结构。正相和反相快速色谱法对脑苷脂的分离有效，快原子轰击质谱（FAB-MS）、核磁共振氢谱（¹H-NMR）、核磁共振碳谱（¹³C-NMR）、双量子滤波相关谱（DQF-COSY）、异核多量子相干谱（HMQC）实验以及化学反应对阐明其结构很有帮助。长链碱和葡萄糖神经酰胺七乙酸酯的二甲基二硫醚衍生物的电子轰击质谱（EI-MS）对于确定长链部分的双键位置起决定性作用。