在小鼠改良局部淋巴结试验中证明的光反应性检测。
Detection of photoreactivity demonstrated in a modified local lymph node assay in mice.
作者信息
Vohr H W, Homey B, Schuppe H C, Kind P
机构信息
Institute of Toxicology, Bayer AG, Wuppertal, Germany.
出版信息
Photodermatol Photoimmunol Photomed. 1994 Apr;10(2):57-64.
Photoallergic and phototoxic reactions are an increasing problem in dermatologic practice. Currently available test models are heterogeneous and do not allow differentiation between photoallergic and phototoxic reactions. We have modified the local lymph node assay to screen for photoreactive compounds. We found an ultraviolet A (UVA) irradiation-dependent increase in the weights and cell counts of the auricular lymph nodes after application of 8-methoxypsoralen, tribromosalicylanilide, trichlorosalicylanilide, chlorpromazine and promethazine. In contrast to these photoreactive compounds, contact sensitizers did not produce any reaction to UVA irradiation in the local lymph node assay. This assay system has the advantage of being less expensive and less time-consuming and might even be able to differentiate between phototoxic, photoallergic and contact allergic reactions.
光变应性和光毒性反应在皮肤科临床实践中是一个日益严重的问题。目前可用的测试模型具有异质性，无法区分光变应性和光毒性反应。我们改进了局部淋巴结试验以筛选光反应性化合物。我们发现，在应用8-甲氧基补骨脂素、三溴水杨酰苯胺、三氯水杨酰苯胺、氯丙嗪和异丙嗪后，耳淋巴结的重量和细胞计数会因紫外线A（UVA）照射而增加。与这些光反应性化合物不同，接触致敏剂在局部淋巴结试验中对UVA照射没有产生任何反应。该检测系统具有成本较低、耗时较少的优点，甚至可能能够区分光毒性、光变应性和接触过敏性反应。
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