Priestley G C, Aldridge R D, Sime P J, Wilson D
Department of Dermatology, University of Edinburgh, Scotland, U.K.
Br J Dermatol. 1994 Jul;131(1):52-6. doi: 10.1111/j.1365-2133.1994.tb08457.x.
The accumulation of glycosaminoglycans in the skin in pretibial myxoedema appears to be a response by local fibroblasts to a stimulating factor in the patient's serum, but the identity of the factor, its ability to stimulate skin fibroblasts as opposed to cultured thyroid cells, and the specificity of its effect to pretibial skin fibroblasts, are all controversial. We have studied fibroblasts cultured from the lesional skin of two women with pretibial myxoedema, and compared their proliferation and secretion of glycosaminoglycans with those of fibroblasts from the patients' forearms and from the forearm skin of two normal subjects. We found that in the presence of the patients' sera all six lines of fibroblasts secreted more glycosaminoglycans [205 +/- 21% (SD)] than with normal human sera (147 +/- 19%), or fetal calf serum (100%). Fibroblast proliferation showed the same pattern of differences: patients' sera 142 +/- 22%; normal human sera 116 +/- 9%, and fetal calf serum 100%. These experiments confirm the presence of a serum factor in pretibial myxoedema which is capable of stimulating the activity of skin fibroblasts in vitro, and show that its effects are not restricted to fibroblasts from pretibial skin or to those grown from the skin of the patients. Proliferation of normal fibroblasts cultured in medium supplemented with fetal calf serum was reduced by Sandostatin (octreotide), but it failed to inhibit their secretion of glycosaminoglycans. In contrast, secretion of glycosaminoglycans by a patient's pretibial skin fibroblasts was almost completely inhibited by 1 mM minoxidil. In the presence of patients' sera Sandostatin (0.1-10 micrograms/ml) reduced secretion of glycosaminoglycans by about 50%.(ABSTRACT TRUNCATED AT 250 WORDS)
胫前黏液性水肿患者皮肤中糖胺聚糖的蓄积似乎是局部成纤维细胞对患者血清中一种刺激因子的反应，但该因子的身份、其刺激皮肤成纤维细胞而非培养甲状腺细胞的能力以及其对胫前皮肤成纤维细胞作用的特异性均存在争议。我们研究了从两名患有胫前黏液性水肿的女性病变皮肤培养的成纤维细胞，并将其糖胺聚糖的增殖和分泌情况与患者前臂及两名正常受试者前臂皮肤的成纤维细胞进行了比较。我们发现，在患者血清存在的情况下，所有六组成纤维细胞分泌的糖胺聚糖[205±21%（标准差）]均多于正常人血清（147±19%）或胎牛血清（100%）。成纤维细胞增殖也呈现相同的差异模式：患者血清为142±22%；正常人血清为116±9%，胎牛血清为100%。这些实验证实了胫前黏液性水肿患者血清中存在一种能够在体外刺激皮肤成纤维细胞活性的因子，并表明其作用并不局限于胫前皮肤的成纤维细胞或患者皮肤来源的成纤维细胞。在添加胎牛血清的培养基中培养的正常成纤维细胞的增殖可被善得定（奥曲肽）抑制，但它未能抑制其糖胺聚糖的分泌。相比之下，1 mM米诺地尔几乎完全抑制了患者胫前皮肤成纤维细胞糖胺聚糖的分泌。在患者血清存在的情况下，善得定（0.1 - 10微克/毫升）可使糖胺聚糖的分泌减少约50%。（摘要截选至250字）
