Cheung H S, Nicoloff J T, Kamiel M B, Spolter L, Nimni M E
J Invest Dermatol. 1978 Jul;71(1):12-7. doi: 10.1111/1523-1747.ep12543646.
Skin fibroblasts from the shoulder and lower extremities of normal individuals, as well as from patients with pretibial myxedema (PTM) were grown in culture. When cells reached the monolayer stage, they were labeled with 3H-glucosamine and tested for hyaluronic acid synthesis in the presence of either serum from PTM patients or normal human serum. All the fibroblasts from the pretibial area synthesized 2 to 3 times more hyaluronic acid when incubated with PTM sera than when incubated in normal human serum. Fibroblasts cultured from skin of the back or prepuce did not respond to PTM sera. This heat-stable, protease-sensitive, and dialyzable, fibroblast-stimulating factor is not a 7S gamma-globulin. The enhanced sensitivity to PTM sera exhibited by fibroblasts from the lower extremities may explain why the lesions in this disease are restricted primarily to that area.
取自正常个体肩部和下肢以及胫前黏液性水肿（PTM）患者的皮肤成纤维细胞在培养中生长。当细胞达到单层阶段时，用³H-葡糖胺进行标记，并在PTM患者血清或正常人血清存在的情况下检测透明质酸合成。与正常人血清相比，来自胫前区域的所有成纤维细胞在与PTM血清一起孵育时合成的透明质酸多2至3倍。从背部皮肤或包皮培养的成纤维细胞对PTM血清无反应。这种热稳定、蛋白酶敏感且可透析的成纤维细胞刺激因子不是7Sγ-球蛋白。下肢成纤维细胞对PTM血清表现出的增强敏感性可能解释了为什么该疾病的病变主要局限于该区域。
