Shoukry M I, el-Khatim M S, Gumaa K A
Department of Biochemistry, Faculty of Medicine, Kuwait University.
Acta Diabetol. 1994 Apr;31(1):40-2. doi: 10.1007/BF00580759.
The effect of lipoprotein-deficient serum (LPDS) with and without added low-density lipoprotein (LDL), isolated from diabetic subjects, on the replication of SV40-transformed islet cells (HIT cells) was investigated. Whole serum as well as LPDS preparations stimulated DNA synthesis maximally when added to the culture medium at a final concentration of 0.1%. The addition of LDL at 25 and 175 micrograms protein/ml medium did not cause further stimulation. On the contrary, the higher concentrations resulted in a significant inhibition. These results suggest that previously observed stimulation of DNA synthesis in smooth muscle cells by LDL from diabetic subjects is most likely due to the presence of growth factors in the serum of these patients and not to LDL per se.
研究了从糖尿病患者分离出的、添加和未添加低密度脂蛋白（LDL）的脂蛋白缺乏血清（LPDS）对SV40转化胰岛细胞（HIT细胞）复制的影响。当以0.1%的终浓度添加到培养基中时，全血清以及LPDS制剂能最大程度地刺激DNA合成。在培养基中添加25和175微克蛋白质/毫升的LDL不会引起进一步的刺激。相反，较高浓度会导致显著抑制。这些结果表明，先前观察到的糖尿病患者LDL对平滑肌细胞DNA合成的刺激很可能是由于这些患者血清中存在生长因子，而非LDL本身。
