Górny D, Korzeniowska J, Marciniak A, Pielecki J
Department of Pathophysiology, Medical Academy, Lublin, Poland.
J Physiol Pharmacol. 1994 Mar;45(1):173-81.
This study was designed to examine the effect of the rat atrial extract (RAE) and synthetic rat ANF123-150 (rANF) on the renal Na, K-ATPase activity in Wistar anesthetized rats. Na, K-ATPase activity was assayed by measuring the amount of inorganic phosphate liberated from ATP. RAE from 40.3, 80.6 mg of tissue and rANF in the following doses: 0.043, 0.087, 0.173, 0.260 nM/kg/min reduced Na, K-ATPase activity by 59, 64 and 11, 34, 37, 45% respectively in the renal medulla but not in the cortex. It was associated with the increase in diuresis and natriuresis. Five and ten minutes after the end of rANF administration, Na, K-ATPase activity was decreased by 78 and 57% respectively, diuresis and natriuresis were significantly higher than the control. After fifteen minutes enzyme activity returned to normal, diuresis and natriuresis were increased. After thirty minutes there was a 37% increase in Na, K-ATPase activity but diuresis and natriuresis were still higher than control values. We conclude that the inhibition of Na, K-ATPase in the medulla of the rat kidney is one of the mechanisms of ANF action.
本研究旨在检测大鼠心房提取物（RAE）和合成大鼠心房钠尿肽123 - 150（rANF）对Wistar麻醉大鼠肾钠钾ATP酶活性的影响。通过测量从ATP释放的无机磷酸量来测定钠钾ATP酶活性。来自40.3、80.6mg组织的RAE以及以下剂量的rANF：0.043、0.087、0.173、0.260nM/kg/min，分别使肾髓质而非皮质中的钠钾ATP酶活性降低59%、64%以及11%、34%、37%、45%。这与利尿和利钠增加相关。在给予rANF结束后5分钟和10分钟，钠钾ATP酶活性分别降低78%和57%，利尿和利钠显著高于对照组。15分钟后酶活性恢复正常，利尿和利钠增加。30分钟后钠钾ATP酶活性增加37%，但利尿和利钠仍高于对照值。我们得出结论，大鼠肾脏髓质中钠钾ATP酶的抑制是心房钠尿肽作用的机制之一。
