Massad G, Arceneaux J E, Byers B R
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.
Biometals. 1994 Jul;7(3):227-36. doi: 10.1007/BF00149553.
Most species of the genus Aeromonas produce the siderophore amonabactin, although two species produce enterobactin, the siderophore of many enteric bacteria. Both siderophores contain 2,3-dihydroxybenzoic acid (2,3-DHB). Siderophore genes (designated aebC, -E, -B and -A, for aeromonad enterobactin biosynthesis) that complemented mutations in the enterobactin genes of the Escherichia coli 2,3-DHB operon, entCEBA(P15), were cloned from an enterobactin-producing isolate of the Aeromonas spp. Mapping of the aeromonad genes suggested a gene order of aebCEBA, identical to that of the E. coli 2,3-DHB operon. Gene probes for the aeromonad aebCE genes and for amoA (the entC-equivalent gene previously cloned from an amonabactin-producing Aeromonas spp.) did not cross-hybridize. Gene probes for the E. coli 2,3-DHB genes entCEBA did not hybridize with Aeromonas spp. DNA. Therefore, in the genus Aeromonas, 2,3-DHB synthesis is encoded by two distinct gene groups; one (amo) is present in the amonabactin-producers, while the other (aeb) occurs in the enterobactin-producers. Each of these systems differs from (but is functionally related to) the E. coli 2,3-DHB operon. These genes may have diverged from an ancestral group of 2,3-DHB genes.
气单胞菌属的大多数菌种会产生铁载体氨单菌素，不过有两个菌种会产生肠杆菌素，即许多肠道细菌的铁载体。这两种铁载体都含有2,3 - 二羟基苯甲酸（2,3 - DHB）。从一株产肠杆菌素的气单胞菌属分离株中克隆到了一些铁载体基因（分别命名为aebC、-E、-B和-A，用于气单胞菌肠杆菌素生物合成），这些基因可互补大肠杆菌2,3 - DHB操纵子entCEBA(P15)中肠杆菌素基因的突变。对气单胞菌基因的定位表明其基因顺序为aebCEBA，与大肠杆菌2,3 - DHB操纵子的顺序相同。气单胞菌aebCE基因和气单胞菌amoA（先前从一株产氨单菌素的气单胞菌属克隆得到的与entC等效的基因）的基因探针不会交叉杂交。大肠杆菌2,3 - DHB基因entCEBA的基因探针与气单胞菌属DNA不杂交。因此，在气单胞菌属中，2,3 - DHB的合成由两个不同的基因组编码；一个（amo）存在于产氨单菌素的菌种中，另一个（aeb）存在于产肠杆菌素的菌种中。这两个系统中的每一个都与大肠杆菌2,3 - DHB操纵子不同（但在功能上相关）。这些基因可能是从一组2,3 - DHB的祖先基因分化而来的。
