Saito K, Horie M, Nakazawa H
Saitama Institute of Public Health, Japan.
J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):270-5. doi: 10.1016/0378-4347(94)00056-5.
Urinary histamine (Him) and 1-methylhistamine (MH) were determined by liquid chromatography (LC) using on-column derivatization coupled with a column-switching technique. An intact urine sample without any purification was applicable to the LC system because all steps for purification and fluorescence derivatization were fully automated. It was observed that the concentrations of Him and MH increased after hydrolysis of the urine, suggesting the presence of conjugated Him and MH. The level of total/free Him in urine was significantly higher in cancer patients than in normal subjects. Further, a significant correlation between Him and MH was observed in the hydrolysed urine of both normal subjects and cancer patients.
采用柱上衍生化结合柱切换技术的液相色谱法（LC）测定尿组胺（Him）和1-甲基组胺（MH）。未经任何纯化的完整尿液样本适用于LC系统，因为纯化和荧光衍生化的所有步骤均完全自动化。观察到尿液水解后Him和MH的浓度增加，提示存在结合型Him和MH。癌症患者尿液中总/游离Him水平显著高于正常受试者。此外，在正常受试者和癌症患者的水解尿液中均观察到Him与MH之间存在显著相关性。
