Dumas F, Aussel C, Pernet P, Martin C, Giboudeau J
Laboratoire de Biochimie A, Hôpital Saint Antoine, Paris, France.
J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):276-81. doi: 10.1016/0378-4347(94)00041-7.
Intestinal permeability can be modified by various illnesses, trauma and sepsis. Alterations of the intestinal wall can facilitate the diffusion of potentially harmful substances such as endotoxins, as well as bacterial translocation. We describe the validation of a capillary gas chromatographic method for the determination of mannitol and lactulose, used as intestinal permeability probes. The method is linear up to 3 g/l for mannitol and 300 mg/l for lactulose; recovery from overload samples is between 92 to 110%. Intra-assay coefficients of variation (C.V.s) were 2.7 and 6.8% for mannitol and lactulose, respectively, and inter-assay C.V.s were 8.9 and 9.3%. Normal values for 25 healthy subjects (mean +/- S.D.) were 14.5 +/- 3.1% and 0.27 +/- 0.15% for mannitol and lactulose, respectively. The GC method presented is rapid and precise.
肠道通透性可因各种疾病、创伤和脓毒症而改变。肠壁的改变可促进内毒素等潜在有害物质的扩散，以及细菌移位。我们描述了一种用于测定甘露醇和乳果糖的毛细管气相色谱法的验证，甘露醇和乳果糖用作肠道通透性探针。该方法对甘露醇的线性范围高达3 g/l，对乳果糖的线性范围高达300 mg/l；过载样品的回收率在92%至110%之间。甘露醇和乳果糖的批内变异系数分别为2.7%和6.8%，批间变异系数分别为8.9%和9.3%。25名健康受试者的正常数值（均值±标准差），甘露醇为14.5±3.1%，乳果糖为0.27±0.15%。所介绍的气相色谱法快速且精确。
