Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, Gdańsk, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Oct 15;907:34-40. doi: 10.1016/j.jchromb.2012.08.031. Epub 2012 Sep 4.
A new analytical procedure was described for the simultaneous determination of lactulose, mannitol and sucrose in urine, in which HILIC chromatography and tandem mass spectrometry detection are used. Sugars are orally administered for the estimation of intestinal permeability in children digestive tract. Samples were purified by dispersive solid phase extraction (d-SPE) using Amberlite MB150 resin. Raffinose was selected as an internal standard. The chosen chromatographic separation was carried out on ZIC(®)-HILIC column in 10 min at a flow rate of 0.3 mL/min, using mixture of acetonitrile (ACN) and ammonium acetate (NH(4)Ac) in water (H(2)O) as the mobile phase. Within-run precision (CV) measured at three concentrations was 1.08%, 0.32% and 0.49% for lactulose; 1.88%, 0.47% and 0.75% for mannitol, 2.95%, 1.31% and 0.6% for sucrose. Between-run CVs were 0.75%, 1.1% and 1.2% for lactulose; 1.1%, 1.02% and 1.01% for mannitol; 1.17%, 1.4% and 1.05% for sucrose. Analytical recovery of all three sugar probes was 95.06-99.92%. The detection limits were: 15.94 ng/mL for lactulose, 17.10 ng/mL for sucrose and 11.48 ng/mL for mannitol. The proposed method is rapid, simple, sensitive and suitable for the determination of intestinal permeability of the sugar derivatives in children.
描述了一种新的分析方法，用于同时测定尿液中的乳果糖、甘露醇和蔗糖，该方法采用亲水作用色谱和串联质谱检测。这些糖被口服用于估计儿童消化道的肠道通透性。样品通过分散固相萃取（d-SPE）用 Amberlite MB150 树脂进行纯化。选择棉子糖作为内标。选择的色谱分离在 ZIC(®)-HILIC 柱上在 10 分钟内以 0.3 mL/min 的流速进行，使用乙腈（ACN）和乙酸铵（NH(4)Ac）在水中（H(2)O）的混合物作为流动相。在三个浓度下测量的日内精密度（CV）分别为乳果糖 1.08%、0.32%和 0.49%；甘露醇 1.88%、0.47%和 0.75%；蔗糖 2.95%、1.31%和 0.6%。日间精密度（CV）分别为乳果糖 0.75%、1.1%和 1.2%；甘露醇 1.1%、1.02%和 1.01%；蔗糖 1.17%、1.4%和 1.05%。所有三种糖探针的分析回收率为 95.06-99.92%。检测限分别为：乳果糖 15.94ng/mL，蔗糖 17.10ng/mL，甘露醇 11.48ng/mL。该方法快速、简单、灵敏，适用于儿童糖衍生物肠道通透性的测定。
