Young S G, Farese R V, Pierotti V R, Taylor S, Grass D S, Linton M F
Gladstone Institute of Cardiovascular Disease, San Francisco.
Curr Opin Lipidol. 1994 Apr;5(2):94-101. doi: 10.1097/00041433-199404000-00005.
Transgenic mice that express human apolipoprotein (apo)B have been developed by microinjecting fertilized mouse oocytes with an 80 kb genomic DNA fragment that encompasses the entire human APOB gene. In the transgenic mice expressing the largest amounts of human apoB, the concentration of human apoB100 in the plasma is nearly as high as the levels observed in normolipidemic humans (50 mg/dl). Virtually all of the human apoB100 in the transgenic plasma is located in the LDL fraction, resulting in substantially increased levels of LDL cholesterol. These human apoB-transgenic mice should be useful animal models for understanding various aspects of lipoprotein metabolism and for further delineating the role of LDL in atherogenesis.
通过将包含整个人载脂蛋白B（apoB）基因的80 kb基因组DNA片段显微注射到受精的小鼠卵母细胞中，已培育出表达人载脂蛋白B的转基因小鼠。在表达量最高的人apoB转基因小鼠中，血浆中人apoB100的浓度几乎与血脂正常的人（50 mg/dl）中观察到的水平一样高。转基因血浆中几乎所有的人apoB100都位于低密度脂蛋白（LDL）组分中，导致LDL胆固醇水平大幅升高。这些人apoB转基因小鼠应是理解脂蛋白代谢各个方面以及进一步阐明LDL在动脉粥样硬化发生中作用的有用动物模型。
