Baretton G B, Valina C, Vogt T, Schneiderbanger K, Diebold J, Löhrs U
Pathologisches Institut, Ludwig-Maximilians-Universität München, Germany.
Cancer Res. 1994 Aug 15;54(16):4472-80.
To gain a better understanding of chromosomal aberrations in direct correlation with histology, we studied tumor material from 35 patients (36 regions) with primary prostate carcinoma by nonisotopic in situ hybridization. Nine biotinylated DNA probes were used on serial paraffin sections (centromer-specific probes for X, Y, 1, 7, 8, 10, 17, and 18, and a telomer-specific probe for 1p; ONCOR). Of the 324 hybridized sections, 94% were suitable for evaluation. In 34 of the 35 cases (35 of 36 regions) 1-8 chromosomal aberrations were detected. Chromosome X showed supernumerary centromer copies in 44% of cases. The probes for chromosomes 1, 1p, 10, and 18 demonstrated deletions in 25, 23, 40 and 58% of cases, respectively. Gains as well as deletions were present for Y, 7, 8, and 17 in 31, 25, 36, and 58% of cases, respectively. In 27% of cases discordant copy numbers of the centromer- and the telomer-specific probes for chromosome 1 were observed. No aberration which might be specific for prostate cancer could be established. The rate of aneusomy increased significantly with histological grade. Intratumoral heterogeneity of chromosomal aberrations was revealed in one case. Due to the higher sensitivity of nonisotopic in situ hybridization, aneusomic cases outnumbered cases with cytometrically determined DNA aneuploidy. In view of published results of metaphase preparations, the high frequency of aneusomy and some of the chromosomal aberrations detected by nonisotopic in situ hybridization were unexpected.
为了更好地理解与组织学直接相关的染色体畸变，我们通过非同位素原位杂交研究了35例原发性前列腺癌患者（36个区域）的肿瘤组织。在连续石蜡切片上使用了9种生物素化DNA探针（针对X、Y、1、7、8、10、17和18号染色体的着丝粒特异性探针，以及针对1p染色体的端粒特异性探针；ONCOR公司）。在324张杂交切片中，94%适合评估。35例患者中的34例（36个区域中的35个）检测到1至8种染色体畸变。X染色体在44%的病例中显示有额外的着丝粒拷贝。1、1p、10和18号染色体的探针分别在25%、23%、40%和58%的病例中显示有缺失。Y、7、8和17号染色体在31%、25%、36%和58%的病例中分别既有增加也有缺失。在27%的病例中观察到1号染色体着丝粒特异性探针和端粒特异性探针的拷贝数不一致。未发现可能是前列腺癌特异性的畸变。非整倍体率随组织学分级显著增加。在1例病例中发现了肿瘤内染色体畸变的异质性。由于非同位素原位杂交的敏感性更高，非整倍体病例的数量超过了通过细胞计数法确定的DNA非整倍体病例。鉴于已发表的中期制备结果，非同位素原位杂交检测到的非整倍体高频率以及一些染色体畸变是出乎意料的。
