Chono Y, Abe H, Iwasaki Y, Nagashima K
Department of Neurosurgery, Hokkaido University School of Medicine, Sapporo, Japan.
Childs Nerv Syst. 1994 Apr;10(3):180-4. doi: 10.1007/BF00301087.
The volume and DNA synthesis of the neuroepithelium in induced myeloschisis in Long-Evans rats as shown by hematoxylin-eosin and BrdU/antiBrdU immunohistochemical staining patterns were examined at different stages of embryonal development. On day 14 of gestation, control animals contained BrdU-incorporating cells mainly at the alar plate of the closed neural tube. On the same day, the everted neural plate of rats with myeloschisis showed active, diffuse uptake of BrdU in the cells of the matrix layer, although an increase in the volume of the everted neural plate was not yet identifiable. On day 21 of gestation, rats with myeloschisis showed a marked increase in the volume of the neuroepithelium compared with controls. Our investigations suggest that, in myeloschisis, more neuroepithelial cells than normal retain their capability for DNA synthesis on day 14 of gestation, and the overgrowth of the neuroepithelium found on day 21 is possibly a secondary effect of failure of neural tube closure.
通过苏木精-伊红染色以及BrdU/抗BrdU免疫组化染色模式，对Long-Evans大鼠诱导性脊髓裂形成过程中神经上皮的体积和DNA合成情况在胚胎发育的不同阶段进行了检查。在妊娠第14天，对照动物中主要在闭合神经管的翼板处含有掺入BrdU的细胞。在同一天，患有脊髓裂的大鼠外翻神经板在基质层细胞中显示出BrdU的活跃、弥漫性摄取，尽管外翻神经板的体积增加尚未可识别。在妊娠第21天，患有脊髓裂的大鼠与对照相比，神经上皮的体积显著增加。我们的研究表明，在脊髓裂中，在妊娠第14天比正常情况更多的神经上皮细胞保留了其DNA合成能力，并且在第21天发现的神经上皮过度生长可能是神经管闭合失败的继发效应。
