Prince J, Deverill C E, Ayliffe G A
J Clin Pathol. 1975 Jan;28(1):71-6. doi: 10.1136/jcp.28.1.71.
A membrane filter was used for assessing the surface disinfecting activity of phenolic disinfectants and a chloroxylenol disinfectant. The influence of the type of organism, inoculum size, and hardness of water was investigated. Pseudomonas aeruginosa was chosen for the standardized test. Disinfectant solutions were prepared in water of 300 ppm hardness and applied for two and a half minutes and eight minutes to the bacteria deposited from filtration of 1 ml of a suspension containing 10-6 bacteria. The membrane filter test has certain advantages over many tests, eg, all organisms surviving after treatment can be counted and residual disinfectant is easily removed.
使用膜滤器评估酚类消毒剂和对氯二甲苯酚消毒剂的表面消毒活性。研究了微生物类型、接种量和水硬度的影响。选择铜绿假单胞菌进行标准化试验。在硬度为300 ppm的水中制备消毒剂溶液,并将其作用于从1 ml含10⁻⁶个细菌的悬浮液过滤后沉积的细菌上2分半钟和8分钟。膜滤器试验相对于许多试验具有某些优势,例如,可以对处理后存活的所有微生物进行计数,并且残留消毒剂易于去除。