Meers P D, Churcher G M
J Clin Pathol. 1974 Apr;27(4):288-91. doi: 10.1136/jcp.27.4.288.
A membrane filtration apparatus of the type employed in water bacteriology was used in the assessment of some antimicrobial drugs. Samples of liquid nutrient medium inoculated with a small number of bacteria, to which dilutions of a drug had been added before incubation and again later, were filtered and the membranes washed and incubated on solid medium. By this means differences between bacteriostatic and bactericidal action were clearly demonstrated. It was shown that staphylococci which had survived exposure to penicillin or gentamicin at levels below their minimum bactericidal concentrations remained fully responsive to the subsequent addition of more of the same drug, while those which had survived exposure to chloramphenicol or clindamycin were not significantly affected by further addition, so that neither of the latter two drugs was bactericidal against the strain of organism used. Bactericidal effects were also demonstrated by benzyl penicillin against Clostridium welchii and ampicillin against Escherichia coli.
一种在水细菌学中使用的膜过滤装置被用于评估一些抗菌药物。接种少量细菌的液体营养培养基样本，在培养前和之后都加入了药物稀释液，将其过滤，然后洗涤膜并在固体培养基上培养。通过这种方法，抑菌作用和杀菌作用之间的差异得到了清晰的证明。结果表明，暴露于低于其最低杀菌浓度的青霉素或庆大霉素后仍存活的葡萄球菌，对随后添加更多相同药物仍有充分反应，而暴露于氯霉素或克林霉素后仍存活的葡萄球菌，进一步添加药物对其没有显著影响，因此后两种药物对所用菌株均无杀菌作用。苄青霉素对产气荚膜梭菌和氨苄青霉素对大肠杆菌也表现出杀菌作用。
