Romeyn J A, Singh L, Drulak M, Cook J
J Immunol Methods. 1975 Feb;6(4):375-84. doi: 10.1016/0022-1759(75)90008-3.
This paper provides evidence that it is possible to prepare facilitating anti-mouse immunoglobulin (that is, anti-mouse immunoglobulin which facilitates complementary lysis of red cells sensitized with mouse-produced haemolysin) which, when injected into mice 24 hours before an injection of sheep red cells, very markedly reduced the number of haemolysin-producing cells detectable in spleen four days later. The diffusion-lysis method was used to recognize this and other anti-Ig's in heterologous antiserum and fractions thereof. The effective antibody was in the gamma2 fraction of antiserum produced in guinea pigs by injecting them with guinea pig red cells sensitized with mouse-produced haemolysin. This method of immunizing was used in order to stimulate the production of antibody against immunoglobulin which had undergone the configurational change characteristically occurring when antibody unites with antigen. The 19S fraction of the antiserum contained inhibiting anti-mouse immunoglobulin (anti-mouse immunoglobulin which inhibits complementary lysis of red cells sensitized with mouse-produced haemolysin) and interfered with immune depression by the gamma2 fraction. It is postulated that the gamma2 fraction induces complementary lysis only of lymphocytes whose surface immunoglobulin receptors have bound antigen and undergone configurational change. It is suggested that facilitating anti-immunoglobulin of the type described is responsible for immune suppression by anti-lymphocyte serum (ALS). Facilitating anti-mouse immunoglobulin was demonstrated in two samples of ALS (anti-mouse) which were active in suppressing graft rejection, but inhibiting anti-mouse immunoglobulin only was found in a sample which was ineffective in suppressing graft rejection.
本文提供的证据表明，制备促进性抗小鼠免疫球蛋白（即促进由小鼠产生的溶血素致敏的红细胞补体溶解的抗小鼠免疫球蛋白）是可能的。在注射绵羊红细胞前24小时将其注入小鼠体内，4天后脾脏中可检测到的产生溶血素的细胞数量会显著减少。采用扩散溶解法识别异源抗血清及其组分中的这种和其他抗Ig。有效抗体存在于豚鼠注射用小鼠产生的溶血素致敏的豚鼠红细胞后产生的抗血清的γ2组分中。采用这种免疫方法是为了刺激针对免疫球蛋白的抗体产生，这种免疫球蛋白在抗体与抗原结合时发生了特征性的构型变化。抗血清的19S组分含有抑制性抗小鼠免疫球蛋白（抑制由小鼠产生的溶血素致敏的红细胞补体溶解的抗小鼠免疫球蛋白），并干扰γ2组分引起的免疫抑制。据推测，γ2组分仅诱导其表面免疫球蛋白受体已结合抗原并发生构型变化的淋巴细胞的补体溶解。有人提出，所述类型的促进性抗免疫球蛋白是抗淋巴细胞血清（ALS）免疫抑制的原因。在两份具有抑制移植排斥活性的ALS（抗小鼠）样品中证实了促进性抗小鼠免疫球蛋白，但在一份无抑制移植排斥作用的样品中仅发现了抑制性抗小鼠免疫球蛋白。
