Kholodenko B N, Westerhoff H V, Brown G C
E.C. Slater Institute, University of Amsterdam, The Netherlands.
FEBS Lett. 1994 Jul 25;349(1):131-4. doi: 10.1016/0014-5793(94)00617-2.
The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzyme intermediate is equal to the concentration of that enzyme intermediate as a fraction of the total enzyme concentration. Together with simple measurements this powerful relation may be used (i) to estimate certain enzyme intermediate levels, in particular the free enzyme concentration, and (ii) to estimate the control coefficients of rate constants and steps.
不同速率常数限制孤立酶稳态速率的程度，可量化为这些常数和基本步骤的控制系数。我们发现，表征消耗特定酶中间体的单向速率的速率常数的控制系数之和，等于该酶中间体的浓度占总酶浓度的比例。结合简单的测量，这个强大的关系可用于：（i）估计某些酶中间体水平，特别是游离酶浓度；（ii）估计速率常数和步骤的控制系数。
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