酶内速率限制分析：速率常数的通量控制系数与单向速率、单个分离酶的速率常数和热力学参数之间的关系。
The analysis of rate limitation within enzymes: relations between flux control coefficients of rate constants and unidirectional rates, rate constants and thermodynamic parameters of single isolated enzymes.
作者信息
Brown G C, Cooper C E
机构信息
Department of Biochemistry and Molecular Biology, University College, London, U.K.
出版信息
Biochem J. 1994 May 15;300 ( Pt 1)(Pt 1):159-64. doi: 10.1042/bj3000159.
Abstract
The extent to which a rate constant or step within an enzyme mechanism limits the net enzyme rate in a particular condition can be quantified as a flux control coefficient. We derive here a number of relations between the control coefficients and the unidirectional rates, rate constants, and thermodynamic parameters of the enzyme. These and other relations are used to suggest a number of methods for experimentally measuring control coefficients within enzymes.
摘要
在特定条件下，酶机制中的速率常数或步骤对净酶促反应速率的限制程度可以用通量控制系数来量化。我们在此推导出了控制系数与酶的单向速率、速率常数和热力学参数之间的一些关系。这些关系以及其他关系被用于提出一些在酶内实验测量控制系数的方法。
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