Sipko T P, Udina I G, Badagueva Iu N, Sulimova G E
Genetika. 1994 Feb;30(2):225-9.
Polymorphism of kappa-casein gene (alleles A and B) in Belovezhsky and Caucasian-Belovezhsky European bisons, bisons and their hybrids, as well as in cattle, yaks, and Yakut cattle was studied by PCR method, followed by restriction analysis. European bisons and yaks, being referred, correspondingly, to genera Bison and Bos of Bovidae family, were stated to have A and B alleles, previously found in cattle (Bos taurus). Yakut cattle was shown to have a new rare F allele of kappa-casein gene with 7.7% frequency. The comparative genetic analysis of the above groups, according to kappa-casein polymorphism, revealed similarity between Belovezhsky and Caucasian-Belovezhsky european bison strains. Observed decrease of A allele frequency in Caucasian-Belovezhsky European bisons must be induced by the high level of inbreeding. Measures preventing the loss of genetic diversity in lines of european bison was proposed.
采用聚合酶链式反应（PCR）方法并结合限制性分析，研究了别洛韦日斯基欧洲野牛、高加索-别洛韦日斯基欧洲野牛、欧洲野牛及其杂种、以及牛、牦牛和雅库特牛中κ-酪蛋白基因（等位基因A和B）的多态性。欧洲野牛和牦牛分别属于牛科的野牛属和牛属，研究表明它们拥有先前在普通牛（Bos taurus）中发现的A和B等位基因。结果显示雅库特牛拥有频率为7.7%的κ-酪蛋白基因新的稀有F等位基因。根据κ-酪蛋白多态性对上述群体进行的比较遗传分析，揭示了别洛韦日斯基欧洲野牛和高加索-别洛韦日斯基欧洲野牛品系之间的相似性。高加索-别洛韦日斯基欧洲野牛中观察到的A等位基因频率下降必定是由高度近亲繁殖导致的。文中提出了防止欧洲野牛品系遗传多样性丧失的措施。
