Udina I G, Badagueva Iu N, Sulimova G E, Zakharov I A
Genetika. 1995 Dec;31(12):1704-6.
The polymerase chain reaction method, followed by restriction fragment length polymorphism analysis (PCR-RFLP), was used to study the alleles of the kappa-casein gene in a sample of European bison (n = 38). On the basis of the distribution of the restriction sites, a new allele kappa-CnG was revealed in the European bison, in addition to the kappa-CnA allele represented in cattle. The nucleotide sequence of an exon 4 fragment of the kappa-CnG allele was obtained. In the population of European bison alleles kappa-CnA and kappa-CnG occurred at frequencies 0.224 and 0.776, respectively.
采用聚合酶链反应方法，随后进行限制性片段长度多态性分析（PCR-RFLP），对欧洲野牛样本（n = 38）中的κ-酪蛋白基因等位基因进行研究。根据限制性酶切位点的分布，除了牛中存在的κ-CnA等位基因外，在欧洲野牛中还发现了一个新的等位基因κ-CnG。获得了κ-CnG等位基因第4外显子片段的核苷酸序列。在欧洲野牛群体中，κ-CnA和κ-CnG等位基因的出现频率分别为0.224和0.776。
