Wang W C, Afromowitz M, Hannaford B
Department of Electrical Engineering FT-10, University of Washington, Seattle 98195.
IEEE Trans Biomed Eng. 1994 Mar;41(3):298-304. doi: 10.1109/10.284952.
This communication proposes an optical method using forward light scattering to measure the deflection of a micropipette from a normal contact force with a small elastic solid, and the damping coefficient of a small fluid sample through a dynamic interaction with the pipette. An analytical and experimental study of this sensor is performed and described for each property. Topics covered include: 1) forward light scattering technique for determining the diameter of a micropipette (using exact and simplified geometric interference theories); 2) deflection measurement and analysis on the pipette-sample system using the finite element method; and 3) equivalent damping coefficient and resonant frequency measurement of the pipette-fluid system and its numerical solution derived from the finite element model. A comparison of the experimental results and the analytical results is included to provide the means for analyzing the potential of this new method.
本通讯提出了一种光学方法，该方法利用前向光散射来测量微量移液器相对于与小弹性固体的法向接触力的偏转，以及通过与移液器的动态相互作用来测量小流体样品的阻尼系数。针对每种特性，对该传感器进行了分析和实验研究并进行了描述。涵盖的主题包括：1）用于确定微量移液器直径的前向光散射技术（使用精确和简化的几何干涉理论）；2）使用有限元方法对移液器 - 样品系统进行偏转测量和分析；3）移液器 - 流体系统的等效阻尼系数和谐振频率测量及其从有限元模型导出的数值解。文中还包括了实验结果与分析结果的比较，以提供分析这种新方法潜力的手段。
