通过直接染色体测序鉴定，spo0F基因的Tn5401破坏导致苏云金芽孢杆菌中CryIIIA过量产生。
Tn5401 disruption of the spo0F gene, identified by direct chromosomal sequencing, results in CryIIIA overproduction in Bacillus thuringiensis.
作者信息
Malvar T, Baum J A
机构信息
Ecogen Inc., Langhorne, Pennsylvania 19047-1810.
出版信息
J Bacteriol. 1994 Aug;176(15):4750-3. doi: 10.1128/jb.176.15.4750-4753.1994.
Abstract
The Bacillus thuringiensis spo0F gene was identified by chromosomal DNA sequencing of sporulation mutants derived from a B. thuringiensis transposon insertion library. A spo0F defect in B. thuringiensis, which was suppressed by multicopy hknA or kinA, resulted in the overproduction of the CryIIIA insecticidal crystal protein.
摘要
苏云金芽孢杆菌spo0F基因是通过对来自苏云金芽孢杆菌转座子插入文库的芽孢形成突变体的染色体DNA测序鉴定出来的。苏云金芽孢杆菌中的spo0F缺陷可被多拷贝的hknA或kinA抑制，该缺陷会导致CryIIIA杀虫晶体蛋白的过量产生。
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