非洲爪蟾卵提取物中精子染色质的重塑：核心组蛋白磷酸化和连接组蛋白B4在染色质组装中的作用
Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
作者信息
Dimitrov S, Dasso M C, Wolffe A P
机构信息
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Cell Biol. 1994 Aug;126(3):591-601. doi: 10.1083/jcb.126.3.591.
Abstract
We find that the remodeling of the condensed Xenopus laevis sperm nucleus into the paternal pronucleus in egg extracts is associated with phosphorylation of the core histones H2A, H2A.X and H4, and uptake of a linker histone B4 and a HMG 2 protein. Histone B4 is required for the assembly of chromatosome structures in the pronucleus. However neither B4 nor core histone phosphorylation are required for the assembly of spaced nucleosomal arrays. We suggest that the spacing of nucleosomal arrays is determined by interaction between adjacent histone octamers under physiological assembly conditions.
摘要
我们发现在卵提取物中，非洲爪蟾精子浓缩细胞核重编程为雄原核与核心组蛋白H2A、H2A.X和H4的磷酸化，以及连接组蛋白B4和一种HMG 2蛋白的摄取有关。组蛋白B4是原核中染色小体结构组装所必需的。然而，间隔核小体阵列的组装既不需要B4也不需要核心组蛋白磷酸化。我们认为，在生理组装条件下，核小体阵列的间距由相邻组蛋白八聚体之间的相互作用决定。
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