Rodgers M, McLoughlin J, Peers N, Anderson J, Woods P, Mitchell G G, Robertson W R
Department of Clinical Biochemistry, Hope Hospital, Salford, UK.
Hum Reprod. 1994 Apr;9(4):638-42. doi: 10.1093/oxfordjournals.humrep.a138562.
We examined the possible contribution of human chorionic gonadotrophin (HCG) in Pergonal to the serum luteinizing hormone (LH)-like bioactivity in 10 patients (median age 32 years, range 28-38) with tubal infertility who were undergoing in-vitro fertilization (IVF), together with 19 controls (median age 30 years, range 21-43). IVF patients were treated with clomiphene (50 mg twice daily) over days 2-6 and Pergonal (150 IU i.m.) daily from day 5 until at least day 10. Serum LH was measured by fluoro-immunometric assay (I-LH) and in-vitro Leydig cell bioassay (B-LH). Serum HCG was measured by fluoro-immunometric assay. The data were analysed by paired two-tailed t-test, following logarithmic transformation. From days 1-5, there was an increase in serum B-LH (mean, 95% confidence intervals given in parentheses) from 8.3 (6.8, 10.2) IU/l to 11.7 (9.8, 13.9) IU/l [P = 0.004], and in serum I-LH from 4.5 (3.7, 5.4) IU/l to 5.4 (4.6, 6.3) IU/l [P = 0.002]. From days 5-8, there was a rise in B-LH to 16.6 (12.6, 21.9) IU/l [P = 0.023]. The rise in I-LH to 6.3 (5.1, 7.8) IU/l [P = 0.081] failed to reach significance. Furthermore, serum HCG was < 0.75 IU/l until after Pergonal was administered on day 5, then rose to a plateau on day 8 at 1.2 (0.8, 1.6) IU/l. Serum HCG in the controls remained < 0.75 IU/l throughout.(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了10名（年龄中位数32岁，范围28 - 38岁）因输卵管性不孕正在接受体外受精（IVF）的患者中，果纳芬（Pergonal）中的人绒毛膜促性腺激素（HCG）对血清促黄体生成素（LH）样生物活性的可能影响，同时设置了19名对照（年龄中位数30岁，范围21 - 43岁）。IVF患者在第2 - 6天接受克罗米芬（50毫克，每日两次）治疗，从第5天开始每天接受果纳芬（150国际单位，肌肉注射）治疗，至少持续到第10天。血清LH通过荧光免疫测定法（I - LH）和体外睾丸间质细胞生物测定法（B - LH）进行测量。血清HCG通过荧光免疫测定法进行测量。数据在对数转换后通过配对双尾t检验进行分析。从第1 - 5天，血清B - LH（括号内给出均值及95%置信区间）从8.3（6.8，10.2）国际单位/升增加到11.7（9.8，13.9）国际单位/升[P = 0.004]，血清I - LH从4.5（3.7，5.4）国际单位/升增加到5.4（4.6，6.3）国际单位/升[P = 0.002]。从第5 - 8天，B - LH上升至16.6（12.6，21.9）国际单位/升[P = 0.023]。I - LH上升至6.3（5.1，7.8）国际单位/升[P = 0.081]未达到显著水平。此外，在第5天给予果纳芬之前血清HCG < 0.75国际单位/升，然后在第8天升至平台期，为1.2（（0.8，1.6）国际单位/升。对照组的血清HCG在整个过程中均保持<< < 0.75国际单位/升。（摘要截短于250字）
