Matsuda Y, Oshio S, Yazaki T, Umeda T, Akihama S
Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
Hum Reprod. 1994 Apr;9(4):664-8. doi: 10.1093/oxfordjournals.humrep.a138568.
This study examined the effect of some proteinase inhibitors on liquefaction of human semen. It revealed that a strong plasmin inhibitor, 6-amidino-2-naphthyl-6-guanidinobenzoate dihydrochloride (Fusan) showed a significant inhibition of liquefaction, while t-amino caproic acid (t-ACA) showed a weak retardation effect. In terms of sperm quality after liquefaction, Fusan (10 mM), ethyl diamine tetra-acetic acid (EDTA) and Urinastatin completely inhibited sperm motility. Fusan (1 mM) and Lima bean trypsin inhibitor (LBTI) decreased sperm motility significantly, while leupeptin and t-ACA had little effect. Leupeptin, LBTI, t-ACA and Fusan (1 mM) did not affect sperm speed. 50% inhibition of sperm motility was calculated to be approximately 1.7 mM of Fusan concentration. In addition, two inhibitors, Chymostatin and Phosphoramidon were also tested with each experiment and had no effect on liquefaction or on sperm motility and speed. These results strongly suggest that plasmin may play an important role in the liquefaction process of human semen.
本研究检测了某些蛋白酶抑制剂对人类精液液化的影响。结果显示，一种强效纤溶酶抑制剂，6-脒基-2-萘基-6-胍基苯甲酸二盐酸盐（富山）对精液液化有显著抑制作用，而ε-氨基己酸（ε-ACA）则表现出较弱的延缓作用。就液化后的精子质量而言，富山（10 mM）、乙二胺四乙酸（EDTA）和乌司他丁完全抑制精子活力。富山（1 mM）和利马豆胰蛋白酶抑制剂（LBTI）显著降低精子活力，而亮抑酶肽和ε-ACA的影响较小。亮抑酶肽、LBTI、ε-ACA和富山（1 mM）不影响精子速度。计算得出，约1.7 mM的富山浓度可使精子活力受到50%的抑制。此外，在每次实验中还测试了两种抑制剂，抑肽酶和磷酰胺脒，它们对精液液化、精子活力和速度均无影响。这些结果有力地表明，纤溶酶可能在人类精液液化过程中起重要作用。
