Upadhyaya M, Hibbard B M, Walker S M
Fertil Steril. 1981 Jun;35(6):657-61. doi: 10.1016/s0015-0282(16)45560-3.
A comparative study evaluating the effect of different mucolytic agents on viscid semen and on sperm motility at successive time intervals was carried out. Sputolysin (dithiothreitol, Calbiochem Behring, 1978) was found to be most efficient in liquefying viscid semen and also in preserving sperm motility. The mean liquefaction times for Sputolysin, Alevaire (a mucolytic detergent), and alpha-amylase were found to be 10 minutes, 39 minutes, and 20 minutes, respectively. The rate of decline in sperm motility over an interval of 24 hours was found to be least with Sputolysin, followed in order by phosphate-buffered saline, alpha-amylase, control, and Alevaire. The adverse effect of Alevaire on sperm motility over successive time intervals was quite marked. A serial dilution study with Sputolysin showed that, at higher dilution (1:80), liquefaction time was prolonged (24 minutes), but it was still shorter than with Alevaire (39 minutes) and nearly the same as with alpha-amylase (20 minutes).
开展了一项比较研究，评估不同黏液溶解剂在连续时间间隔对黏稠精液和精子活力的影响。发现痰溶解素（二硫苏糖醇，卡尔 Biochem 贝林公司，1978 年）在液化黏稠精液以及保持精子活力方面最为有效。痰溶解素、阿莱维（一种黏液溶解洗涤剂）和α-淀粉酶的平均液化时间分别为 10 分钟、39 分钟和 20 分钟。发现在 24 小时的时间间隔内，精子活力下降率以痰溶解素最低，其次依次为磷酸盐缓冲盐水、α-淀粉酶、对照和阿莱维。阿莱维在连续时间间隔对精子活力的不利影响相当明显。用痰溶解素进行的系列稀释研究表明，在较高稀释度（1:80）时，液化时间延长（24 分钟），但仍短于阿莱维（39 分钟），与α-淀粉酶（20 分钟）相近。
