Aiyathurai E J, Makinde V, Huang C L, Das R E, Zaidi M, Moonga B S
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London, UK.
Mol Cell Biochem. 1994 Feb 9;131(1):61-6. doi: 10.1007/BF01075725.
Generalised metabolic and electrolyte disturbances are known to accompany both plasma and surface virus infections. We have investigated whether these infections could impair the transport of Ca2+ from cells under conditions of controlled concentrations of the energy substrate glucose. Thus, cytosolic calcium levels ([Ca2+]i) were measured in single isolated lymphocytes obtained from healthy volunteers or those suffering from coryza. Before making measurements using a Ca(2+)-sensitive fluorescent dye indo 1, we incubated lymphocytes in buffers containing 0 mM-, 5.6 mM- or 11.2 mM-[glucose]. We found that [Ca2+]i of lymphocytes obtained from the sick were significantly higher than those from healthy controls both at 0 mM and 5.6 mM-[glucose], and that [Ca2+]i was inversely related to the media glucose concentration for both groups. These results suggest a diminished capacity of cation pumping in viral infections, such as coryza, in relationship to the available glucose as energy substrate.
已知全身性代谢和电解质紊乱与血浆及体表病毒感染相伴。我们研究了在能量底物葡萄糖浓度受控的条件下，这些感染是否会损害细胞内Ca2+的转运。因此，我们测量了从健康志愿者或患感冒者中分离出的单个淋巴细胞的胞质钙水平（[Ca2+]i）。在用Ca(2+)敏感荧光染料indo 1进行测量之前，我们将淋巴细胞置于含有0 mM、5.6 mM或11.2 mM - [葡萄糖]的缓冲液中孵育。我们发现，在0 mM和5.6 mM - [葡萄糖]条件下，患病者淋巴细胞的[Ca2+]i显著高于健康对照组，并且两组的[Ca2+]i均与培养基葡萄糖浓度呈负相关。这些结果表明，在诸如感冒等病毒感染中，与作为能量底物的可用葡萄糖相关的阳离子泵功能有所减弱。
