Waller R L, Brattin W J, Dearborn D G
Life Sci. 1984 Aug 13;35(7):775-81. doi: 10.1016/0024-3205(84)90347-3.
Lymphocytes prepared from normal individuals and patients with cystic fibrosis (CF) were compared with regard to intracellular Ca2+ concentration, distribution, and handling. No difference between control and CF was found in the concentration of cytosolic free Ca2+ (98 +/- 5 vs 102 +/- 7 nM), and no difference was observed in the kinetics with which control and CF cells restored cytoplasmic Ca2+ toward normal following a perturbation induced by cold-exposure. However, total intracellular Ca2+ is about 25% higher in CF lymphocytes than in control. Of this excess Ca2+, about 50% appears to be sequestered in mitochondria. This suggests that some difference in Ca2+ handling does exist, but the significance of this in cystic fibrosis remains to be determined.
对从正常个体和囊性纤维化（CF）患者体内分离出的淋巴细胞，在细胞内钙离子浓度、分布及处理方面进行了比较。对照细胞与CF细胞的胞质游离钙离子浓度无差异（分别为98±5 nM和102±7 nM），并且在冷暴露诱导的扰动后，对照细胞与CF细胞恢复细胞质钙离子至正常水平的动力学方面也未观察到差异。然而，CF淋巴细胞中的总细胞内钙离子比对照细胞高约25%。在这些过量的钙离子中，约50%似乎被隔离在线粒体中。这表明在钙离子处理方面确实存在一些差异，但这在囊性纤维化中的意义仍有待确定。
