Brunner D, Dücker K, Oellers N, Hafen E, Scholz H, Klämbt C
Zoologisches Institut, Universität Zürich, Switzerland.
Nature. 1994 Aug 4;370(6488):386-9. doi: 10.1038/370386a0.
The fate of the R7 photoreceptor cell in the developing eye of Drosophila is controlled by the Sevenless (Sev) receptor tyrosine kinase. Sev activates a highly conserved signal transduction cascade involving the proteins Ras1 and Raf and the Rolled/mitogen-activated protein (Rl/MAP) kinase. Here we show that the ETS domain protein encoded by the P2 transcript of the pointed (pnt) gene is a nuclear target of this signalling cascade which acts downstream of Rl/MAP kinase. The PntP2 protein is phosphorylated by Rl/MAP kinase in vitro at a single site and this site is required for its function in vivo. Furthermore, we present genetic and biochemical data suggesting that MAP kinase controls neural development through phosphorylation of two antagonizing transcription factors of the ETS family, Yan and PntP2.
果蝇发育中眼睛里R7光感受器细胞的命运由Sevenless（Sev）受体酪氨酸激酶控制。Sev激活一个高度保守的信号转导级联反应，该反应涉及蛋白质Ras1、Raf以及Rolled/丝裂原活化蛋白（Rl/MAP）激酶。我们在此表明，由尖状（pnt）基因的P2转录本编码的ETS结构域蛋白是此信号级联反应的一个核靶点，其作用于Rl/MAP激酶的下游。PntP2蛋白在体外被Rl/MAP激酶在单个位点磷酸化，且该位点是其在体内发挥功能所必需的。此外，我们提供的遗传学和生物化学数据表明，MAP激酶通过磷酸化ETS家族的两个拮抗转录因子Yan和PntP2来控制神经发育。
