[Influence of autolysis on the immunohistology of tissue-bound immunglobulins in the kidney (author's transl)].

The influence of autolysis on the immunhistological detectability and the immunological properties of tissue-bound basement membrane antibodies were investigated in the rat kidney. The immunhistological demonstration of antibodies was not affected up to 3 days post mortem. The disturbing autofluorescence of the autolytic tissue can be avoided by using peroxydase labeled antibodies. Basement-membrane antibodies that were eluted from the kidneys after an autolysis period of 2 days showed a homogenous linear deposition in kidney basement membrane after reinjection into normal rats. Their antibody properties were not affected.