Bergenbrant S, Yi Q, Osby E, Osterborg A, Ostman R, Björkholm M, Holm G, Lefvert A K
Department of Internal Medicine, Karolinska Hospital, Stockholm, Sweden.
Scand J Immunol. 1994 Aug;40(2):216-20. doi: 10.1111/j.1365-3083.1994.tb03453.x.
The prevalence of peripheral blood B cells secreting antibodies reacting with the F(ab')2 fragment of monoclonal IgG was studied in five patients with multiple myeloma (MM), nine patients with monoclonal gammopathy of undetermined significance (MGUS) and six healthy controls. An enzyme-linked immunospot assay allowed direct visualization of antibody producing B cells. All patients had B cells producing antibodies to autologous or allogeneic monoclonal IgG. Autoreactive cells were found more frequently than alloreactive cells in seven out of nine patients with MGUS and three out of four patients with MM. The same frequency of alloreactive cells in the patient groups was detected in healthy individuals. These findings show the existence of B cells producing anti-idiotypic antibodies which could be a part of an idiotypic network in monoclonal gammopathies.
我们研究了5例多发性骨髓瘤（MM）患者、9例意义未明的单克隆丙种球蛋白病（MGUS）患者和6名健康对照者外周血中分泌与单克隆IgG的F(ab')2片段发生反应的抗体的B细胞的患病率。酶联免疫斑点试验可直接观察产生抗体的B细胞。所有患者均有产生针对自身或同种异体单克隆IgG抗体的B细胞。在9例MGUS患者中的7例以及4例MM患者中的3例中，自反应性细胞比同种异体反应性细胞更常见。在健康个体中检测到患者组中同种异体反应性细胞的频率相同。这些发现表明存在产生抗独特型抗体的B细胞，这可能是单克隆丙种球蛋白病中独特型网络的一部分。
