Wimalasena J
Department of Obstetrics/Gynecology, University of Tennessee Medical Center, Knoxville 37920.
Alcohol Clin Exp Res. 1994 Apr;18(2):369-74. doi: 10.1111/j.1530-0277.1994.tb00027.x.
Clinical observations indicate that ethanol (EtOH) consumption has significant detrimental effects on pregnancy. However, there is a paucity of information on effects of EtOH on human placental function. We have used JEG choriocarcinoma cells that have many of the functional capabilities of syncytiotrophoblasts, as a model to study direct effects of EtOH on placental function. Between 20-100 mM EtOH decreased rate of cell growth by 25%, but no decrease in [35S]methionine incorporation into protein was noted. EtOH decreased in a dose-dependent manner the secretion of progesterone (P4) in response to cAMP when added with cAMP or when cells were pretreated with EtOH for 2 days. But after 4 or 6 days of pretreatment with EtOH, the P4 response to cAMP was increased by EtOH. Furthermore, EtOH increased the stimulation of P4 secretion by Forskolin. The development of this response was dependent on the period of exposure to EtOH. EtOH also increased estradiol (E2) secretion by unstimulated JEG cells in a dose- and time-dependent manner and increased cAMP stimulated E2 secretion > 2-fold following 4 days of pretreatment with EtOH. These results suggest that EtOH may directly alter hormone secretion by placental cells and such perturbations of endocrine function of the placenta may be responsible for some of the effects of EtOH on pregnancy.
临床观察表明，摄入乙醇（EtOH）对妊娠有显著的有害影响。然而，关于EtOH对人胎盘功能影响的信息却很少。我们使用具有许多合体滋养层细胞功能特性的JEG绒毛膜癌细胞作为模型，来研究EtOH对胎盘功能的直接影响。在20 - 100 mM浓度之间，EtOH使细胞生长速率降低了25%，但未观察到[35S]甲硫氨酸掺入蛋白质的量减少。当与环磷酸腺苷（cAMP）一起添加或细胞用EtOH预处理2天时，EtOH以剂量依赖的方式降低了对cAMP的孕酮（P4）分泌。但是在用EtOH预处理4天或6天后，EtOH增加了对cAMP的P4反应。此外，EtOH增强了福斯高林对P4分泌的刺激作用。这种反应的发展取决于暴露于EtOH的时间。EtOH还以剂量和时间依赖的方式增加了未受刺激的JEG细胞的雌二醇（E2）分泌，并且在用EtOH预处理4天后，增强了cAMP刺激的E2分泌超过2倍。这些结果表明，EtOH可能直接改变胎盘细胞的激素分泌，而胎盘内分泌功能的这种紊乱可能是EtOH对妊娠产生某些影响的原因。
