Asano M, Nio N, Ariyoshi Y
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Agric Biol Chem. 1991 Mar;55(3):825-8.
It has been suggested that peptide inhibitors of prolyl endopeptidase (PEP) may act as anti-amnestic agents. In the hope of finding PEP inhibitors in milk proteins, we synthesized a total of 37 human beta-casein peptide fragments containing proline residues. It was found that the peptides with PEP inhibition activity in vitro were located in the region of amino acid residues 49-59 of human beta-casein. The most potent inhibitor was Ile-Tyr-Pro-Phe-Val-Glu-Pro-Ile (IC50 = 8 microM).
有人提出脯氨酰内肽酶（PEP）的肽类抑制剂可能具有抗遗忘作用。为了在乳蛋白中找到PEP抑制剂，我们总共合成了37个含有脯氨酸残基的人β-酪蛋白肽片段。结果发现，体外具有PEP抑制活性的肽位于人β-酪蛋白氨基酸残基49 - 59区域。最有效的抑制剂是异亮氨酸-酪氨酸-脯氨酸-苯丙氨酸-缬氨酸-谷氨酸-脯氨酸-异亮氨酸（IC50 = 8微摩尔）。
