Fukuda M, Yasukochi Y, Kikuchi Y, Nagata Y, Kimbara K, Horiuchi H, Takagi M, Yano K
Department of Agricultural Chemistry, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Jul 29;202(2):850-6. doi: 10.1006/bbrc.1994.2008.
The nucleotide sequence of the upstream region of the bphC gene from Pseudomonas sp. strain KKS102 was determined. Four genes were found in this region. Deduced amino acid sequences of the first, second, third and fourth genes showed significant homology with a large subunit of iron-sulfur protein, a small subunit of iron-sulfur protein, ferredoxin and dihydrodiol dehydrogenase, respectively, from other bacteria which degrade biphenyl/polychlorinated biphenyls, toluene and benzene. E. coli, in which the four genes, bphC and the gene for ferredoxin reductase from benzene degrading bacterium were expressed, was able to produce meta-cleavage compounds from chlorinated biphenyls. These results show that these gene products are functional in both biphenyl and polychlorinated biphenyls degradation.
测定了假单胞菌属菌株KKS102中bphC基因上游区域的核苷酸序列。在该区域发现了四个基因。第一、第二、第三和第四个基因推导的氨基酸序列分别与其他降解联苯/多氯联苯、甲苯和苯的细菌中的铁硫蛋白大亚基、铁硫蛋白小亚基、铁氧还蛋白和二氢二醇脱氢酶具有显著同源性。在其中表达了这四个基因、bphC基因和来自苯降解细菌的铁氧还蛋白还原酶基因的大肠杆菌，能够从多氯联苯产生间位裂解化合物。这些结果表明，这些基因产物在联苯和多氯联苯的降解中均具有功能。
