里氏非血红素铁加氧酶系统咔唑1,9a -双加氧酶中铁氧化还原蛋白还原酶组分的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of the ferredoxin reductase component in the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase.
作者信息
Ashikawa Yuji, Uchimura Hiromasa, Fujimoto Zui, Inoue Kengo, Noguchi Haruko, Yamane Hisakazu, Nojiri Hideaki
机构信息
Biotechnology Research Center, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):499-502. doi: 10.1107/S174430910702163X. Epub 2007 May 12.
Abstract
Carbazole 1,9a-dioxygenase (CARDO), which consists of an oxygenase component (CARDO-O) and the electron-transport components ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R), catalyzes dihydroxylation at the C1 and C9a positions of carbazole. CARDO-R was crystallized at 277 K using the hanging-drop vapour-diffusion method with the precipitant PEG 8000. Two crystal types (types I and II) were obtained. The type I crystal diffracted to a maximum resolution of 2.80 A and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 158.7, c = 81.4 A. The type II crystal was obtained in drops from which type I crystals had been removed; it diffracted to 2.60 A resolution and belonged to the same space group, with unit-cell parameters a = b = 161.8, c = 79.5 A.
摘要
咔唑1,9a -双加氧酶（CARDO）由一个加氧酶组分（CARDO - O）以及电子传递组分铁氧还蛋白（CARDO - F）和铁氧还蛋白还原酶（CARDO - R）组成，催化咔唑C1和C9a位的双羟基化反应。使用含有沉淀剂聚乙二醇8000的悬滴气相扩散法，在277 K条件下使CARDO - R结晶。获得了两种晶体类型（I型和II型）。I型晶体的衍射极限分辨率为2.80 Å，属于空间群P4(2)2(1)2，晶胞参数a = b = 158.7，c = 81.4 Å。II型晶体是从已去除I型晶体的液滴中获得的；其衍射分辨率为2.60 Å，属于同一空间群且晶胞参数a = b = 161.8，c = 79.5 Å。
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